Leica SP8 DLS Laser Scanning Confocal SPIM

Confocal and SPIM in one

Location

CIID, BSL2, EG, room 025

Objectives

  • 63x/1.4 HC PL APO CS2 Oil
  • 63x/1.3 HC PL APO CORR CS2 Gycerol
  • 20x/0.75 HC PL APO IMM CORR CS2 H20, Glycerol, Oil
  • 2.5x/0.07 HC PL FLUOTAR (for SPIM)
  • 25x/0.95 HC FLUOTAR L W (for SPIM)

Purposes

  • Environment control
  • Resonant scanner
  • Adaptive Focus Control (AFC)
  • Spectral Detection
  • Acusto-ooptical beam splitter (AOBS)
  • HyD dectors

Lasers

  • UV (50mW) – 405nm
  • Argon (65mW) – 458,476,488, 496, 514nm
  • DPSS Yellow (20mW) – 561nm
  • HeNe (10mW) – 633nm

 

Book microscope

Leica SP8 TCS DLS Laser Scanning Confocal SPIM

Links

Materials and Methods template

Point laser scanning confocal microscopy was performed on a Leica SP8 microscope using HC PL APO CS2 63x/1.4 N.A. oil immersion objective. Live-cell imaging was performed at 37˚C and 5% CO2.