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Core Facility – FFCF Flow Cytometry and FACS Core Facility

Flow cytometry and FACS (fluorescence activated cell sorting) represent fast and reliable methods for characterizing and isolating certain cell subpopulations based on fluorescent labeling, and can be applied to a great variety of organisms and cell types, including both prokaryotic and eukaryotic cells.

The facility is equipped with state-of-the-art instruments and offers the possibility of flow cytometric cell analysis and cell sorting.

The FFCF is a DFG-registered service unit (RI 00566). The facility is mainly supported by the ZMBH. Financial support was also provided by the Field of Focus 1 / HMLS, CellNetworks, SFB 638, SFB 1036 and the GK 1188. Dr. Jochen Barths is the interim Head of the FACS Core Facility, stepping in for Dr. Monika Langlotz, who normally runs the FFCF as head of the facility together with Prof. Dr. Michael Knop as responsible PI.

Project Request

All researchers of the University of Heidelberg, DKFZ, EMBL and MPImF are welcome to request service and instrument training from the facility. We also accept projects from non-campus scientists.  

We offer introduction tutorials to flow cytometry and the use of the analytical instruments, as well as support and aid in all questions concerning flow cytometry as a method. Users, who have received the necessary training, can autonomously operate the analyzers. The facility staff operates the cell sorters and provides aid in using the analyzers.

Trained users have access to our online booking system. To arrange an individual instrument training or to request a service appointment for cell analysis or sorting please send an e-mail to facs-core-facility@zmbh.uni-heidelberg.de

Instrument Booking

SERVICES & EQUIPMENT

Equipment

Flow Cytometers
Cell Sorters
Flow Cytometry Software

Further Information on our Equipment

Team

Tabelle

	Dr. Jochen Barths (interim Head) Im Neuenheimer Feld 345 EG R066 (Office) and R009/R010 (Lab) 69120 Heidelberg +49 6221 - 546843 j.barths@zmbh.uni-heidelberg.de Jochen works part-time, but should be always reachable in person between 1-3 pm.
	Dr. Monika Langlotz (Head) Im Neuenheimer Feld 345 EG R066 (Office) and R009/R010 (Lab) 69120 Heidelberg +49 6221 - 546843 m.langlotz@zmbh.uni-heidelberg.de
	Claudia Helbig (TA) +49 6221 - 546843

Selected publications

Tabelle

Constitutional PIGA mutations cause a novel subtype of hemochromatosis in patients with neurologic dysfunction. Muckenthaler L, Marques O, Colucci S, Kunz J, Fabrowski P, Bast T, Altamura S, Höchsmann B, Schrezenmeier H, Langlotz M, Richter-Pechanska P, Rausch T, Hofmeister-Mielke N, Gunkel N, Hentze MW, Kulozik AE, Muckenthaler MU. Blood. 2022 Mar 3;139(9):1418-1422.
Glypican-1 drives unconventional secretion of fibroblast growth factor 2. Sparn C, Dimou E, Meyer A, Saleppico R, Wegehingel S, Gerstner M, Klaus S, Ewers H, Nickel W. Elife. 2022 Mar 29;11:e75545.
Balancing the length of the distal tip by septins is key for stability and signalling function of primary cilia. Kanamaru T, Neuner A, Kurtulmus B, Pereira G. EMBO J. 2022 Jan 4;41(1):e108843.
Constitutive oxidants from hepatocytes of male iPLA2β-null mice increases the externalization of phosphatidylethanolamine on plasma membrane. Wong HY, Langlotz M, Gan-Schreier H, Xu W, Staffer S, Tuma-Kellner S, Liebisch G, Merle U, Chamulitrat W. Free Radic Res. 2021 Jun;55(6):625-633.
Tissue-specific transcriptome profiling of the Arabidopsis inflorescence stem reveals local cellular signatures. Shi D, Jouannet V, Agustí J, Kaul V, Levitsky V, Sanchez P, Mironova VV, Greb T. Plant Cell. 2021 Apr 17;33(2):200-223.
High throughput screening of novel AAV capsids identifies variants for transduction of adult NSCs within the subventricular zone. Kremer LPM, Cerrizuela S, Dehler S, Stiehl T, Weinmann J, Abendroth H, Kleber S, Laure A, El Andari J, Anders S, Marciniak-Czochra A, Grimm D, Martin-Villalba A. Mol Ther Methods Clin Dev. 2021 Jul 16;23:33-50.
Co-chaperone involvement in knob biogenesis implicates host-derived chaperones in malaria virulence. Diehl M, Roling L, Rohland L, Weber S, Cyrklaff M, Sanchez CP, Beretta CA, Simon CS, Guizetti J, Hahn J, Schulz N, Mayer MP, Przyborski JM. PLoS Pathog. 2021 Oct 6;17(10):e1009969.

Funding Resources

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