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IDIP Services & Equipment

Biosafety Level 2 Instruments

Leica SP8 TCS DLS Confocal and SPIM in one

Table

  • 63x/1.4 HC PL APO CS2 Oil
  • 63x/1.3 HC PL APO CORR CS2 Gycerol
  • 20x/0.75 HC PL APO IMM CORR CS2 H2O, Glycerol, Oil
  • 2.5x/0.07 HC PL FLUOTAR (for SPIM)
  • 25x/0.95 HC FLUOTAR L W (for SPIM)
  • Environment control
  • Resonant scanner
  • Adaptive Focus Control (AFC)
  • Spectral Detection
  • Acusto-optical beam splitter (AOBS)
  • HyD detectors
  • UV (50mW) - 405nm
  • Argon (65mW) - 458, 476, 488, 496, 514nm
  • DPSS Yellow (20mW) - 561nm
  • HeNe (10mW) - 633nm

Location: CIID, BSL2, EG, room 025

Materials and Methods for this Device

Abberior Instruments MINFLUX

Table

  • UPLXAPO60XO 1.42 Oil
  • UPLSAPO 60XW 1.2 Water
  • UPLSAPO 60XS2 1.3 Silicone
  • Isotropic molecular localization precision of 4nm
  • Active sample stabilization in xyz
  • Drift correction with the beamline monitoring system
  • 6 APD detectors
  • 2 color MINFLUX imaging
  • 3 MINFLUX lasers with beam shaping
  • 488nm, 561nm, 642nm
  • Activation laser - 405nm

Location: Bioquant building, INF267, EG

Further Information on MINFLUX

cryoCLEM Workflow Instrumentation

Location: CIID, BSL2, EG Room 046 and 051

Features:

  • Leica EM ICE for high pressure freezing
  • GP2 for grid plunging
  • AFS2 for freeze substitution
  • cryoCLEM light microscope
  • UC7/FC7 cryo-ultramicrotome

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