Nikon Imaging Center Equipment & Services
The Nikon Imaging Center offers a variety of microscopic systems, covering a wide range of applications.
Access to microscopes is given after training so that trained users can perform their experiments independently. Only the Light sheet Ultramicroscope and the 2-photon TrimScope have to be operated by NIC staff because of their complexity.
In addition to microscopes, the NIC has analysis workstations (running Nis-Elements, FIJI) and a server running Huygens HRM for deconvolution.
Table
Confocal | |
A1R | Confocal microscope of pointscanning type. With additional resonant scanner and 32-channel spectral detector. 6 laser lines available - Room U36 |
AX | Confocal microscope of pointscanning type. 6 laser lines available - Room U36 |
Ti-VoX | Confocal microscope of the spinning disk type. For high resolution/high magnification imaging of single cells. Allows acquisition of two channels simultaneously - Room U15 |
Ti-Crest | Confocal microscope of the spinning disk type. With 7 laser lines. Also suitable for lower resolution/lower magnification imaging - Room U29 |
TriM-Scope | Two photon confocal microscope with photomultiplier and CCD signal detection - Room U14 |
Superresolution | |
N-SIM | Confocal + widefield microscope, super resolution microscope of the structured illumination type - Room U29 |
Widefield | |
TIRF | Total Internal Reflection Fluorescence microscope with widefield and epifluorescence capabilities + FRAP unit - Room U15 |
NiE | Upright widefield research microscope with epifluorescence and DIC + a low noise high resolution color CMOS camera for histological stainings - Room U15 |
Ti-HCS | Advanced widefield high content screening with brightfield and epifluorescence illumination - Room U29 |
SMZ25 | Widefield stereo zoom microscope with 0.5x and 2x long working distance optics plus a 25x zoom range. With epifluorescence illumination. Ultra high sensitive b/w-camera and a medium resolution color camera - Room U13 |
SMZ1270 | Stereo microscope with epifluorescence illumination - Room U03 |
Lightsheet | |
LaVision Ultrascope II | Light sheet microscope for big (optically cleared) specimen - Room U30 |
Luxendo InVi-SPIM | Light sheet microscope for small samples and live imaging - Room U03 |
For 2D/3D super-resolution microscopy (localization precision 2nm – 5nm ) and fast tracking (10kHz), please visit the CCTP associated technology network.
Image Analysis
Image data can be analyzed by our user in the computer room U04. Currently we are in the process of upgrading to new analysis computers, so that at the moment only two workstations are available for our users. Since we do not have a position for an image analysis specialist, we can only offer limited help with data analysis. The following programs are available:
- NIS-Elements AR (Nikon)
- Volocity (PerkinElmer)
- Huygens (SVI)
-
ImageJ/Fiji (open source)
